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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
Omega Bio-Tek/E.Z.N.A.® FastFilter Plasmid Maxi Kit/25-preps/D6924-03
产品编号:D6924-03
市  场 价:¥7364.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$368.20
品      牌: Omega Bio-Tek
公      司:OmegaBioTek
公司分类:
Omega Bio-Tek/E.Z.N.A.® FastFilter Plasmid Maxi Kit/25-preps/D6924-03
商品介绍

Overview

The E.Z.N.A.® FastFilter Plasmid Maxi Kit combines the convenience of spin columns with the speed of syringe filters to ease large-scale plasmid preparations. The system utilizes Lysate Clearance Filter Syringes instead of centrifugation to rapidly clear bacterial lysates post-alkaline lysis and follows a simple “bind-wash-elute” procedure to deliver high-quality plasmid DNA. The yields vary according to plasmid copy number, E. coli strain, and growth conditions. 50-200 mL of bacterial cultures in LB medium typically produces 0.6-1.2 mg of high copy number plasmid DNA. Up to 500 mL of culture may be processed when working with low copy number plasmid. The system uses centrifugation or vacuum technology for plasmid purification and eliminates the time-consuming gravity-flow columns that require alcohol precipitation. Purified plasmid DNA is suitable for automated fluorescent DNA sequencing (typical reads exceed 800 bp), restriction enzyme digestion, ligation, PCR, in vitro transcription, transformation, and other applications.

  • Speed – Purification of plasmid DNA < 40="">
  • Safety – No phenol/chloroform extractions
  • Versatile – Spin and vacuum formats available
  • High quality – DNA is suitable for a variety of downstream applications

Specifications

For Research Use Only. Not for use in diagnostic procedures.

FeaturesSpecifications
Downstream ApplicationCloning, sequencing, transformation, PCR, restriction digestion, ligation, in vitro transcription etc.
Starting material50-200 mL LB culture with OD600 between 2 and 3; or equivalent
Plasmid typeHigh-copy, low-copy, cosmid DNA
Processing modeManual (centrifugation or vacuum)
Throughput1 - 24
DNA binding technologySilica Maxi Spin Column
Lysate clearance methodFiltration using Lysate clearance syringe
Processing time<40>
Yield600-1200 µg for high copy-number; 50-300 µg for low copy-number
Special noteLysate clearance syringe for rapid sample processing

Protocol and Resources

Product Documentation & Literature

PROTOCOL

D6905 D6924 E.Z.N.A. FastFilter Plasmid DNA Midi & Maxi Kit

SDS

D6924 SDS

SALES SHEET

Product Data

Processing time of plasmid DNA isolation kits

Figure 1.  Total time in minutes required for plasmid DNA isolation from pelleting the bacterial cultures to eluting the plasmid using Omega Bio-tek E.Z.N.A.® FastFilter Plasmid Maxi Kit and comparable products from other competitors.

Citations

View Citations
  • Simultaneous nitrogen and carbon removal from swine digester liquor by the Canon process and denitrification Zhaoji Zhang, Yuanyuan Li, Shaohua Chen, Shumei Wang, Xiaodan Bao
Size

FREE SAMPLE, 2 preps, 25 preps, 100 preps

Format

Maxiprep

品牌介绍

欧米茄Bio-Tek


自1998年成立以来,Omega Biotek就一直在核酸纯化的前列。


自1998年成立以来,Omega Bio-tek通过提供用于临床和基础研究,生物技术和农业应用的产品,一直处于核酸纯化的最前沿。DNA和RNA提取是众多下游分析的第一步,有效而干净的核酸分离至关重要。我们的目标是提供高质量的产品,以帮助您改善工作流程。


为了满足您所有提取需求的最佳选择,我们提供了多种核酸提取化学方法,包括磁珠,硅胶膜和盐析法。这三种不同的方法可用于各种不同的套件和配置中,总共用于900多种产品。我们的第二代HiBind®二氧化硅基质具有极大的灵活性,并提供了从多种来源分离核酸的解决方案。我们的EZ核酸(EZNA®)系统采用旋转柱形式,利用了HiBind®硅胶膜,可从组织,培养的细胞,凝胶和溶液中纯化和回收DNA和RNA。我们的Mag-Bind®技术为使用磁珠分离核酸提供了灵活而可靠的方法。我们的磁珠是针对每种应用定制的,提供最佳的磁化响应时间,凝固和粘结能力。我们的产品种类繁多,从植物的RNA纯化到干血斑的DNA提取。


产品列表:



没有。

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规格

1个

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20次准备

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250 µL×5准备

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500微升


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