Overview
Many high-throughput applications, such as sequencing and genotyping, require the input DNA concentration to be within a certain range for optimal results. Traditionally, a tedious process of quantification, calculation and concentration adjustment must be carried out to normalize the DNA samples.
The Mag-Bind® EquiPure Normalizer Kit completely eliminates the need to quantify and aliquot DNA, saving time, quantification bias and tip cost. Using our proprietary Mag-Bind® Normalizer Beads and binding buffer system, input DNA of various quantities is simply bound, washed and eluted to a final normalized product. The magnetic beads have a limited binding capacity and therefore allow a predefined amount of DNA to be captured and eluted.
The Mag-Bind® EquiPure Normalizer Kit is fully automatable on multiple liquid handling platforms including Hamilton STAR, Tecan Fluent™, Caliper Sciclone, and Beckman Coulter Biomek instruments.
The Mag-Bind® EquiPure Normalizer Kit allows the user to adjust the quantity of DNA that binds to the magnetic beads and elution volumes can be adjusted to vary DNA concentrations to users needs.
- DNA output concentrations: +/- 10%
- No quantification required
- Normalizes genomic DNA or NGS libraries
Specifications
For Research Use Only. Not for use in diagnostic procedures.
| Features | Specifications |
|---|---|
| Downstream application | Application requires gDNA normalization |
| Elution volume | 25-100 µL |
| Starting material | Genomic DNA |
| Starting amount | 2 µg or more |
| DNA recovered | gDNA normalized within 10% range |
| Processing mode | Automated; Manual |
| Throughput | 96 samples per run |
| DNA binding technology | Magnetic beads |
Protocol and Resources
Product Documentation & Literature
PROTOCOL
M6423 Mag-Bind® EquiPure gDNA Normalization Kit
SDS
M6423 SDS
SALES SHEET
APPLICATION NOTE
Genomic DNA Normalization Using Omega Bio-tek’s Mag-BIND® EquiPure gDNA Normalization Kit
Product Data
Figure 1 Title
Figure 1. Varying amounts of 16S NGS libraries were prepared and normalized with the Mag-Bind® EquiPure Normalization Kit. DNA yield was quantified using Promega QuantiFluor® dsDNA system.
Figure 2 Title
Figure 2. Varying amounts of Illumina TruSeq Stranded mRNA libraries were prepared and normalized with the Mag-Bind® EquiPure Normalization Kit. DNA yield was quantified using Promega QuantiFluor® dsDNA system.
Figure 3 Title
Figure 3. Genomic DNA was isolated from whole blood with the Mag-Bind® Blood and Tissue DNA HDQ 96 Kit. Varying amounts were inputted into the different reactions, then normalized with the Mag-Bind® EquiPure gDNA Kit. DNA yield was quantified using Promega QuantiFluor® dsDNA system.
